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德國EMFRET公司專注心血管和血液系統研究用抗體研發(fā)和生產，供應的抗體包括清除血液中血小板、流式細胞術鑒定分析小鼠血小板表面糖蛋白，EMFRET公司的抗體不僅適用于流失分析、WB檢測，還可用于免疫組化/免疫熒光及免疫共沉淀檢測。
| Antibodies for Plaet Depletion in Mice |
# R300 |
|
Product Information |
|
Catalog Number: | R300 (lot # M) |
Isotype: | Rat IgG (Wistar) |
Contents: | 0.5 mg purified immunoglobulins in PBS, sterile filtered |
Concentration: | 0.5 mg/ml |
For research use only, not for diagnostic or therapeutic use. This product is no medical device.
Specificity: This antibody preparation contains a mixture of purified rat monoclonal antibodies
directed against mouse GPIba (CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent plaet depletion in mice1,2. Plaet depletion is frequently used to study the general role of plaets in (patho-) physiology3.
Preparation and Storage: The antibodies were purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography and contain < 0.00025 ng/µg (< 0.0025 EU/µg) protein endotoxin. Stable for six months from date of shipment when stored at 4°C. KEEP STERILE, the preparation contains no preservative. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.
Usage: This preparation is optimized to reduce the plaet count by >95% within 60 minutes after intravenous injection of 2 µg/g bodyweight. It is recommended to inject the antibodies in a volume of 100-200 µl (diluted with sterile PBS).
Depletion of mouse plaets by anti-GPIba treatment
Wild type mice received 2 or 4 µg/g plaet depletion antibodies in 200 µl sterile PBS intravenously and plaet counts were determined at the indicated time points. Results are mean ± s.d. of 6 mice per group.
| R300 | Antibodies for Mouse Plaet Depletion | polyclonal anti-GPIb alpha | Rat IgG | purif. | plaet depletion | 0.5 mg |
其它產品信息如下：
貨號 | 抗原類型 | 克隆方式 | 表型 | 產品形式 | 應用 | 規(guī)格 |
C301 | 清除小鼠血小板 | polyclonal non-immune rat immunoglobulins | Rat IgG | 純化 | 清除小鼠血小板 | 0.5 mg |
D200 | 雙色分析血小板活性 | Wug.E9 JON/A | Rat IgG2b/IgG1 | FITC、PE | 流式細胞術 | 2 x 1.0 ml; 200 tests |
M011-0 | GPVI | JAQ1 | Rat IgG2a | 純化 | IP, IHC, WB | 0.1 mg |
M011-1 | GPVI | JAQ1 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
M021-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg |
M021-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
M022-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.A1 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
M023-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | JON/A | Rat IgG2b | PE | FC (activ. form) | 1.5 ml; 300 tests |
M024-0 | Integrin alphaIIb (GPIIb, CD41) | MwReg30 | Rat IgG1 | purif. | IP, IHC, generation of Fab fragments | 6 mg |
M025-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | purif. | IP, IHC | 0.5 mg |
M025-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
M025-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | PE | FC, IHC | 1.5 ml; 300 tests |
M025-3 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | DyLight 649 | FC, IHC | 1.5 ml; 300 tests |
M030-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.A5 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
M031-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | purif. | IP, IHC, WB | 0.5 mg |
M031-1 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
M040-0 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | purif. | IP, IHC | 0.5 mg |
M040-1 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
M040-2 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | PE | FC, IHC | 1.5 ml; 300 tests |
M040-3 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | DyLight 649 | FC, IHC | 1.5 ml; 300 tests |
M041-0 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | purif. | FC, IHC | 0.5 mg |
M041-1 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
M042-0 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg |
M042-1 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
M043-0 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | purif. | IP, IHC, block | 0.5 mg |
M043-1 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
M050-0 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
M050-1 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
M051-0 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
M051-1 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
M060-1 | GPV (CD42d) | Gon.C2 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
M061-1 | GPV (CD42d) | Gon.G6 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
M070-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg |
M070-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
M071-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | purif. | IP, IHC | 0.5 mg |
M071-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
M075-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1, Sam.G4, Sam.H9 | Rat IgG2b/IgG1 | purif. | IP, IHC | 0.5 mg |
M080-0 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
M080-1 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
M110-0 | CD9 | Nyn.H3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
M110-1 | CD9 | Nyn.H3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
M120-0 | CD31 (PECAM-1) | Pec.H3 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg |
M130-0 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
M130-1 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
M130-2 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | PE | FC, IHC | 1.5 ml; 300 tests |
P140-1 | fibrinogen | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests |
P150-1 | von Willebrand Factor (vWF) | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests |
P180-1 | negative control | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.0 ml; 200 tests |
P190-1 | negative control | polyclonal | Rat IgG | FITC | FC, IHC | 1.0 ml; 200 tests |
P190-2 | negative control | polyclonal | Rat IgG | PE | FC, IHC | 1.0 ml; 200 tests |
| ||||||
P190-3 | negative control | polyclonal | Rat IgG | DyLight 649 | FC, IHC | 1.0 ml; 200 tests |
R300 | Antibodies for Mouse Plaet Depletion | polyclonal anti-GPIb alpha | Rat IgG | purif. | plaet depletion | 0.5 mg |
X488 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 488 | in vivo | 100 µg |
X649 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 649 | in vivo | 100 µg |
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